|AFFILIATIONS||Associate Professor of Neurobiology|
Measuring and controlling neural activity in the retina
Neurons in the retina communicate using electrical and chemical signals. We use a combination of optical, electrophysiological, and molecular methods to study ion channels, the proteins that generate electrical signals, and synaptic transmission, the process that allows a neuron to communicate chemically with other cells. Many of our most recent studies utilize novel chemical reagents, designed to manipulate or monitor the function of ion channels and synapses.
Optical studies of synaptic transmission in the retina
Remote control of neural activity with “light-activated” ion channels
Banghart, B., Borges, K., Isacoff, E., Trauner, D., and Kramer, R.H. (2004). Light-activated ion channels for remote control of neuronal firing. Science (submitted).
Rea, R., Li., J., Dharia, A., Levitan, E.S., Sterling, P., and Kramer, R.H. (2004). Streamlined synaptic vesicle cycle in cone photoreceptor terminals. Neuron 41:755-766.
Krajewski, J.L, Luetje, C.W., and Kramer, R.H. (2003). Tyrosine phosphorylation switches off Ca2+/calmodulin inhibition in rod cyclic nucleotide-gated channels. Journal of Neuroscience 23, 10100-10106.
Molokanova, E., Krajewski, JL., Satpaev, D, Luetje, CW, and Kramer, RH (2003). Subunit contributions to phosphorylation–dependent modulation of rod cyclic nucleotide-gated channels. Journal of Physiology 552: 345-356.
Trivedi, B. and Kramer, R.H. (2002). Patch cramming reveals the mechanism of ling-term suppression of cyclic nucleotides in intact neurons. Journal of Neuroscience 22: 8819-8826.
Savchenko, A., Kraft, T.W., Molokanova, E. and Kramer, R.H. (2001). Growth factors regulate phototransduction in retinal rods by modulating cyclic nucleotide-gated channels through dephosphorylation of a specific tyrosine residue. Proceedings of the National Academy of Sciences, USA 98:5880-5885.
Kramer, R.H. and Molokanova, E. (2001). Modulation of cyclic nucleotide-gated channels and regulation of phototransduction. Journal of Experimental Biology 204:2921-2931.
Molokanova, E., Savchenko, A., and Kramer, R.H. (2000). Interactions of cyclic nucleotide-gated channel subunits and protein tyrosine kinase probed with genistein. Journal of General Physiology 115: 685-696.