VS Student, Austin Roorda lab
Monday, January 25, 2021
11:10 am - 12:30 pm
Structural and Functional Retinal Imaging Using Actively Stabilized Adaptive Optics Optical Coherence Tomography and Adaptive Optics Scanning Laser Ophthalmoscopy
One of the main obstacles for high-resolution retinal imaging is eye motion which causes image artifacts that require extensive post processing to correct. Here, we have implemented real-time, active eye motion corrected adaptive optics optical coherence tomography (AOOCT) by using an adaptive optics scanning laser ophthalmoscope (AOSLO) to correct for the eye ocular aberrations and track the eye motion to actively guide the AOOCT. We utilized a Badal optometer to adjust the focus of the AOOCT independently for imaging the nerve fiber layers and ganglion cells while maintaining a high fidelity photoreceptor mosaic in the AOSLO for active motion correction. We have implemented an AOOCT system with active eye tracking that has full control for focus optimized targeted imaging in 3 dimensions. The AOOCT offers high throughput as individual volume images require minimal distortion correction or lateral registration throughout an imaging session.
By using the complex field obtained from the AOOCT interferogram, we measured the physiological activity of the photoreceptors with nm scale accuracy and recorded the elongation of the cone outer segment in response to a stimulus. By quantifying the physiological changes, we were able to identify cones belonging to different spectral sub-classes, and we confirmed the result over multiple datasets acquired in separate occasions.