Protocols

General

Computer Use and Data

  • Back up your data (burn a CD and keep it at the lab or have two computers with your data)
  • Lab books, data and special equipment ALWAYS stay in the lab (There are no exceptions)
  • Leave no trace behind when leaving the lab (except for above).
    • That means clean up after yourself

Webcalendar

Tutorial
Other Documentation

Lab equipment

How to label solutions [PDF]

Cleaning Up

Colour codes of surgical instruments

Orders

Animal Experiments

Report the following chicken conditions [PDF]

Optical Devices (Lenses and Goggles)

  • How to apply optical devices
  • How to clean optical devices
  • What to do with damaged optical devices

How to setup a cage and your experiment [PDF]

Chick and chick room maintenance [PDF]

How to sacrifice your chickens [PDF]

Needles and Sutures Guidelines [PDF]

Choroid casts [PDF]

Choroid culture [PDF]

RPE Disection [PDF]

RPE Cell Culture Protocol [PDF]

ERG in Chicks [PDF]

Oral Drug Delivery (Lavage) [PDF]

Subbing Slides [PDF]

Setting up the Nikon Coolpix Camera on the Surgery Microscope

 

Measurements

Chick Autorefractor [PDF]

  • Troubleshooting the Autorefractor

How to use the Aberrometer

How to use the Corneal Topographer

How to use the Tonometer

How to use the osmometer

IR keratometer Protocol [PDF]

Light Meter

Radio Photometry [PDF]

Ultrasound

Histology/Immunology/BrdU Lableing

BrdU [PDF]

BrdU Immuno Labling [PDF]

BrdU Retinal Labling [PDF]

Cresyl Violet Staining [PDF]

Common Media [PDF]

DAPI Staining [PDF]

ECMA & Chat immunohistochemistry protocols [PDF]

Fisher fixation protocol for retina [PDF]

Fluro Jade [PDF]

ZENK [PDF]

Useful References

Anatomy of Domestic Birds by Nickel Et al...1977 [pdf]

Anatomy of Domestic Chickens and Birds by Koch et al...1973 [pdf]

Baumel Handbook of Avian Anatomy 1993 [pdf]

Chicken Albino Genetics (pp109-67) [pdf]

 

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